The pair were appearing on Uruguayan TV show Por la Camiseta for a light-hearted interview covering subjects such as their favourite films and foodstuffs when the conversation turned to toilet habits.
"What? Do you pee sitting?" Messi asked Suarez, who confirmed that he does before the Argentine admitted: "Me too."
Messi then added: "It's more comfortable. You wake up in the morning, sleepy, and you put the seat down."
Messi and Suarez have found the back of the net a combined 69 times for the Spanish giants this season.