England levelled the scores after the break through Wayne Rooney, but a late strike from Suarez earned his side all three points.
Find out how the action unfolded below.
6.30pmGood evening everyone! Uruguay and England will be hoping to get their World Cup campaigns back on track by claiming three crucial points in Sao Paulo. After opening day defeats, the two nations are playing catch up in Group D in their bids to reach the knockout stages.
6.33pmOne of the shocks of the tournament so far was Uruguay's 3-1 defeat against Costa Rica. Edinson Cavani had given Uruguay the lead, but second-half goals from Joel Campbell, Oscar Duarte and Marco Urena earned the Costa Ricans a memorable victory. England also suffered disappointment as they fell to a 2-1 reverse against Italy. Claudio Marchisio's long-range effort was quickly cancelled out by Daniel Sturridge's goal, but a header from Mario Balotelli shortly after half time gave the Azzurri all three points.
6.36pmA look at the group table shows Costa Rica surprisingly leading the way, with Italy in second place by virtue of goal difference. With those two teams meeting tomorrow afternoon, it is safe to say England and Uruguay cannot afford to make any more mistakes. It would mean that a loss today for either side would put them out of the tournament if Italy and Costa Rica finish level in their clash. I can reveal that both managers have named their teams for today's encounter, so let's take a look at the players who will be starting.
6.45pmTEAM NEWS: There are no changes for England, with Roy Hodgson naming the same lineup that started in their opening match against Italy. Meanwhile, Luis Suarez does start for Uruguay after sitting out their opening Group D loss. Manager Oscar Tabarez makes a total of five changes to his team, with Alvaro Pereira, Jose Maria Gimenez, Nicolas Lodeiro and Alvaro Gonzalez coming into the side.
6.48pmWell, what can we make of those lineups? It seems as if Rooney may play a more central role for England tonight, after his performance on the left against Italy was criticised. If he does play in his more favoured position, then it seems likely Sturridge will move over to the left and he should be able to offer Baines more protection down the wing.
6.52pmThe big headline coming from the South American's team is the inclusion of Luis Suarez. The Liverpool striker has been struggling for fitness after undergoing knee surgery in May, but with his side on the verge of heading out of the tournament, his return will hand them a huge boost. It remains to be seen just how fit he is, but he will be a danger that Cahill and Jagielka will have to watch at all times.
6.55pmUruguay were forced into a couple of changes this evening. Maxi Pereira is suspended after his red card against Costa Rica, while captain Diego Lugano picked up an injury earlier this week. It means that Jose Maria Gimenez and Alvaro Pereira come into the defence. Nicolas Lodeiro and Alvaro Gonzalez should add more steel to a midfield that was overrun in their first match.
6.58pmOpening World Cup defeats are rarely looked upon in a positive light, but there was plenty for England manager Roy Hodgson to be encouraged by following the game in Manaus. His team looked to take the game to the Italians from the start and put in a far more progressive display than shown in their penalty shootout defeat against the same team at the Euros two years ago.
7.01pmA clear bright spark for the English was the form of Raheem Sterling. The 19-year-old was only winning his fifth cap for his country, but looked every bit the established international. He almost scored with a wonderful strike in the opening five minutes that many in the ground thought had found the net, while his pass to Wayne Rooney for the equaliser was a touch of class. Hodgson certainly made the right call handing him a start and there is every chance he can give Godin and the rest of the defence a torrid time this evening.
7.04pmRooney may have come in for some criticism for his display, but his cross to set up Daniel Sturridge's goal was perfectly placed. He didn't get into the game as much as he would have liked and should probably have scored with an effort in the second half. It didn't help that he was forced to play out wide, with much of best play coming through the middle, but his detractors will point to the fact that he has now gone nine World Cup matches without finding the net.
7.07pmDespite some impressive moments in the attack, England found themselves overrun by the Italian midfield, with Andrea Pirlo and Daniele De Rossi instrumental throughout the 90 minutes. There were also clear problems on the left-hand side of the pitch, with Leighton Baines not given enough support. The Italians do boast one of the best midfields in international football and against the Uruguayans Steven Gerrard can expect to dictate the tempo of the match in his deeper-lying role.
7.10pmPhil Jagielka and Gary Cahill were defensively sound and kept Mario Balotelli at bay for much of the game. The Milan striker only needed one moment to make the difference though, as he got above Cahill at the back post to power a header past Joe Hart. Against the likes of Cavani and Suarez, they will need to be alert to make sure that they don't repeat the same mistakes.
7.13pmEngland may have been able to take heart from their defeat, but the same cannot be said of Uruguay. Their second-half collapse against Costa Rica has seen their chances of making it out of the group stages fall drastically and this is now a must-win encounter for them.
7.16pmThe build up to the tournament for Uruguay has been dominated by the fitness of Luis Suarez. The Liverpool man has only recently returned to full training after knee surgery in May and did not play in his team's opener. The fact that he did not enter the pitch at 3-1 down and with his team in desperate need of inspiration tells me that he is far from being fully fit and that playing him tonight will be a huge gamble by Oscar Tabarez.
7.19pmCavani's display was probably the one plus side of the match for Uruguay and even he did not have his best game. His first-half penalty should have been the catalyst for a dominant performance and three points, but a lacklustre showing from his teammates followed. He had one further chance with a header that was well saved in a second half that largely passed him by. Forlan also struggled in the attack and it seems like his best days are behind him.
7.22pmIt would be harsh to lump much of the criticism on the forwards, as the likes of Egidio Arevalo Rios and Walter Gargano in the centre of the pitch failed to link midfield and attack. Cristian Rodriguez and Cristian Stuani also cut frustrated figures and struggled to make their impact felt on the match. It is no surprise to see Gargano and Stuani dropped, as winning the midfield battle is likely to decide which team comes out on top.
7.25pmTaking the most heat following the match is deservedly the back four. Lugano and Godin were badly caught out by the pace of Joel Campbell, which will give huge encouragement to the likes of Sturridge and Sterling. Martin Caceres did not have his best match at left back, while Maxi Pereira got himself suspended for today's game after being sent off for a shocking late challenge on Campbell.
7.28pmPrior to tonight's clash, England have met Uruguay in the World Cup finals twice before. It was the South Americans who were triumphant in the 1954 tournament in Switzerland, where Walter Winterbottom's team suffered a 4-2 defeat. They met again in the opening game of the 1966 World Cup and played out a goalless draw. Both teams made it out of their group, with Uruguay losing 4-0 against West Germany in the quarter-finals. England, of course, went on to win the final 4-2 against the Germans, in what is their sole success in the tournament.
7.30pmTheir last meeting was in a friendly in 2006, when Peter Crouch and Joe Cole scored late on to give England a 2-1 win. Overall, the South Americans have won four of the 10 matches between the sides, with England victorious on three of those occasions.
7.33pmEngland manager Roy Hodgson has just confirmed to ITV Sport that Wayne Rooney will be playing through the middle, with Raheem Sterling going out wide. He was also keen to talk about the trust that he has in the team and says that his players are full of experience and believe in their own abilities.
7.36pmThe stadium that the teams will be playing in tonight is the Arena Corinthians, which was used to hold the opening match where Brazil beat Croatia in a rather controversial encounter. It has been specifically built for the World Cup like many of the stadiums in this tournament and is the home of Brazilian giants Corinthians.
7.39pmTemporary seats have been added for the tournament to allow over 60,000 spectators into the ground, but they will be taken out shortly after the tournament ends. The build up to England's match against Italy was heavily focused on the state of the pitch in Manaus, but we should have no such problems here this evening. One thing that could be on England's side this evening is the weather, as the conditions here at Sao Paulo are much cooler than they faced last week.
7.42pmAn interesting statistic about Uruguay coming into this match is that they haven't come from behind to win a game at the World Cup since 1966. That is certainly an encouraging fact for Hodgson to consider and I would expect England to start the game strongly and put Uruguay under intense pressure.
8 - England have scored in each of their last eight World Cup encounters with South American sides. Character. #ENG
7.45pmOn a somewhat more pessimistic note, only four of 46 sides to lose their opening group game since 1998 have made it through to the knockout stages. They include Spain, who went on to win the tournament four years ago, Ukraine and Ghana in 2006 and eventual semi-finalists Turkey in 2002.
7.50pmPREDICTION: The opening group defeats for both these teams has set up tonight's match for what should be a fascinating encounter. England must feel that they can get at Uruguay's defence, which was badly exposed by the Costa Ricans last week. The Three Lions must also make sure that there are no lapses in concentration at the back, which will surely be pounced upon by the classy Cavani. I certainly see goals in this match and I am going to go for a 2-2 draw, with everything still to play for going into next week's final group games.
7.53pmIt seems that not everyone is happy with the team that Hodgson has sent out this evening. Former England striker Michael Owen believes that Ross Barkley should have got the nod. He got a run out against Italy and showed flashes of what he can do, but despite what impact he makes in this world cup, the Everton man can be a key player for his country in the years to come.
Can understand Roy Hodgson's decision not to change his starting 11 but I still think he should play Ross Barkley.
7.54pmWe are just under five minutes away from kick off and both sets of players are making their way of the tunnel to a rapturous applause at the Arena Corinthians.
7.57pmThe national anthems have been played, with fans of both countries making their voices heard around the ground. As soon as we get the final pre-match formalities out of the way, this crucial clash will be kicking off!
1 minKICKOFF! Uruguay take the kick off and get the match underway at the Arena Corinthians!
2 minPositive start from England who are nearly in when Baines slides a ball through for Welbeck, but it is just beyond the forward's reach. He is nearly given a chance though, as Muslera spills the ball, but the keeper manages to gather at the second attempt.
5 minCHANCE! Uruguay have a sight of goal when Suarez whips in a corner from the left that curls goalwards and almost beats Hart, but the keeper manages to push the ball away at his front post. The ball ends up deflecting behind for another set piece, but this time England clear the threat.
7 minGreat chance for England now, as Sterling shows his skill to cut inside of Lodeiro, before he is fouled by Rodríguez just outside the box. Baines is standing over the free kick...
8 minBaines whips in the free kick, but it is easy for the Uruguay defence to clear. They eventually find Suarez running down the right, but Cahill is across quickly to stop the threat.
9 minYELLOW CARD! Godin picks up the first booking of the evening for a handball on the edge of the box. The free kick is in a wonderful position for England and Rooney looks the favourite to strike it.
10 minCHANCE! It's almost brilliant from Rooney. The striker curls in a wonderful effort that looks as if it's heading into the top corner, but the ball goes agonizingly just past the post. Muslera was getting nowhere near that.
12 minEngland are seeing plenty of the ball inside their opponents' half and they will be encouraged by the start they have made. Suarez has looked busy for the Uruguayans, but we are yet to see him giving Cahill or Jagielka any real problems so far.
15 minCHANCE! Rodriguez has the first big opportunity of the night for Uruguay when he is picked out in acres of space on the left hand side of the box. He decides to take the shot on, but his powerful drive across Hart goes narrowly over the bar.
18 minEngland come forward again and Sturridge wins a corner when his deflected effort flies over the bar. Gerrard takes the resulting corner, but his delivery is poor and headed clear by the defence. That is certainly an area that they could improve on, with the Uruguayans giving away a number of cheap set pieces.
21 minThe game is yet to really spark into life, with brief flashes of skill from each side so far. England do seem to be building a lot of their attacks down the right, with Johnson keen to get forward and link up with Sterling.
24 minUruguay are looking slightly disjointed in attack at the moment and Cavani is struggling to get into the match. His strike partner Suarez hasn't been able to make any decisive runs in behind the defence, with England holding a solid line.
26 minUruguay win a free kick on the far left-hand side of the pitch which is take by Suarez. They have plenty of men forward, but the delivery is far too long and easy for Hart to claim.
27 minCHANCE! Just after mentioning how quiet Cavani had been, the striker is almost in behind, but Cahill comes across to beat him to the ball and ends up putting it out for a corner. The resulting set piece is then pulled across the box for Cavani, who ends up firing his effort over the bar.
29 minControversial moment here as Godin brings down Sterling, but is not punished by the referee. The offence was surely worth of a yellow card and since he has already been booked, his team would have had to play over an hour with ten men.
31 minOFF THE POST! England come within inches of taking the lead! They win a free kick on the touchline down the left that is whipped in by Gerrard. His cross finds Rooney at the back post but the striker heads his effort against the woodwork from just a yard out.
34 minIt is England who are asking the questions in an attacking sense and the Uruguayans are certainly not coping well with the energy and pace of their opponents' front four. They have seen slightly more of the ball in the midfield over the past few minutes, but they are still finding it hard to get their own forward players into the match.
39 minSuarez has hardly been in the game but he has shown that you can't give a player of his quality a glimmer of a chance. The Uruguayans break quickly with Cavani being picked out towards the left hand side of the box. He looks up and sees Suarez arriving in the middle and his cross is inch-perfect, with the Liverpool forward placing a header into the corner past the despairing dive of Hart.
41 minCHANCE! Good response from England as Sturridge bursts into the box, before working some space and firing in a strike that Muslera pushes behind.
43 minEngland are ending the half strongly as they seek to level the scores before the break. They win a string of corners down the right that are taken by Baines, but his deliveries are poor, with Uruguay easily clearing. It would be harsh if England went into the break behind after a promising half of football.
45 minWe are going to have one minute of stoppage time at the end of the half.
8.48pmAt half time in Sao Paulo it is Uruguay who lead thanks to Suarez's late headed goal. All the talk before the match was focused on the Liverpool striker and he showed his undoubted class. England must believe that they can get back into this match, after dominating for large spells, but they were unable to take their chances.
8.52pmThe statistics show that England have seen plenty of the ball. In fact, they have had 60% of the possession and made most of the running. They have also had five attempts compared to Uruguay's three strikes on goal, but all that matters is the one effort on target that has found the back of the net.
1 - Luis Suarez has scored with the first shot on target in the match. Nod.
8.56pmWhat can we say about Wayne Rooney after that first 45 minutes? His brilliant free kick was only inches away from finding the net early on and he hit the post midway through the half. His run of nine games without scoring at the World Cup looks like continuing unless his luck changes drastically after the break.
9pmEngland manager Roy Hodgson must surely be thinking about what he can do to get back into this match. Barkley and Lallana seem the most likely of the players on the bench to come on. Lambert and Wilshere are also options, but as things stand, England's stay at this World Cup is on the verge of coming to an early end.
9.01pmThe players are back out on the pitch and the second half is just about to begin. There have been no changes from either manager.
46 minEngland kick off and get the second 45 minutes underway.
47 minUruguay start the half on the front foot and Suarez attempts to pick out Cavani at the back post, with a similar ball that the PSG forward set up the goal with. This time though, Jagielka spots the run and sees the ball out of play for a thrown in.
49 minCHANCE! Suarez attempted to score from a corner in the first half, and he comes close again after the break. He fires in a powerful effort that beats Welbeck at the front post, but Hart gets back and manages to beat the ball away.
52 minCHANCE! England have started the second half poorly and Cavani should score his side's second after being slipped into the box. He is one-one-one with Hart, but can't direct his curling effort into the far corner of the goal, with the ball heading past the post.
54 minCHANCE! Big, big chance for Rooney. Baines does well to get to the byline and whips in a low ball across the box. It misses Welbeck at the front post, but falls kindly to Rooney in the middle, who then drills in a low strike that is beaten away by Muslera.
55 minBaines is starting to have an impact on this match and he receives the ball again out wide on the left. He then whips in a similar ball to his cross moments ago, but it is just too close to the keeper who bravely claims at the feet of Welbeck.
59minIt looks like England are going to make a change with Barkley readying himself. Meanwhile, a promising moment in the attack sees Henderson steal the ball in the final third, but his cross looking for Welbeck at the back post is hit too long and is claimed by Muslera.
61 minWe have some worrying scenes here with Pereira down following a clash with Sterling. He is being looked at by the medics now and hopefully this isn't too serious.
62 minPereira is thankfully back on his feet, but he might be heading off the pitch. England also wait to make their substitution and it seems like Sterling will be making way.
64 minSUBSTITUTION: Barkley does finally make his entrance onto the pitch in place of Sterling.
67 minSUBSTITUTION: Uruguay have made their first chance of the evening, with Stuani coming on to replace Lodeiro.
68 minYELLOW CARD! Gerrard becomes England's first player of the evening to pick up a booking following a crunching tackle on Rodriguez.
69 minEngland have looked promising coming forward, but far too often their final ball has let them down. Their latest attack sees Rooney link up well with Johnson down the right, but as the full back bursts into the box his touch is poor and the move breaks down.
71 minSUBSTITUTION: It's another change for England, with Lallana coming on in place of Welbeck.
72 minSigns of frustration are creeping into England's play as another attack breaks down out wide on the left. It had looked promising when Sturridge brilliantly took down a crossfield pass from Johnson, but the South Americans were quick to cut out the threat.
74 minSturridge has a half-chance as he turns two Uruguayan defenders 25 yards out from goal, but his low effort is far too central to cause any problems for Muslera.
75 minHe's finally done it! A record of nine games without scoring in the World Cup has come to an end for Rooney! The goal comes when Johnson bursts down the right, before showing great skill to drive into the box. He then slides a low ball across the middle for Rooney to tap in at the back post. What a huge moment for England and their star man.
78 minCHANCE! England are full of confidence now and Sturridge does well to turn two defenders inside the box, before working the ball onto his right foot. He then tries to curl a low effort into the corner, but Muslera does well to parry the ball, before collecting at the second time of asking.
79 minSUBSTITUTION: Uruguay have made another change, with Fucile replacing Gonzalez.
82 minThe game has swung in Uruguay's favour and they are pushing England back deep inside their own half. Suarez has managed to get in down the left a couple of times for his side, but Cahill and Jagielka are holding firm at the back.
84 minHis fitness has been in doubt for weeks, but Suarez's quality has been unquestionable this evening. A long pass forward is flicked back towards his own goal by Gerrard and Suarez is quick to latch onto the ball. He takes a touch to get the ball out of his feet, before lashing a fierce effort past Hart that gives the keeper no chance.
87 minSUBSTITUTION: Lambert is on for England in place of Henderson.
88 minEngland have a good chance to get the ball into the box after winning a free kick out wide on the left. It is Gerrard who takes the set piece, but his delivery is far too long and the opportunity goes to waste.
88 minSUBSTITUTION: Uruguay make their final change of the match, with Suarez heading off for Coates.
90 minWe are going to have five minutes of stoppage time.
90+3 minEngland keep pressing for the vital equaliser, but the Uruguayans have everyone back behind the ball. They end up working some space for Gerrard to shoot from range, but the captain's effort on goal is well wide of the target.
90+4 minIt just does not look like happening for England at the moment as they keep pumping long balls into the box without success. They will have a corner though, after Rooney sees his cross deflected behind.
90+4 minHart comes up for the corner, but it comes to nothing, with a goal kick given. Uruguay are just moments away from picking up all three points this evening.
9.52pmIt is heartbreak for England, as Suarez's two goals have given Uruguay a vital win this evening. England can still qualify, but with two loses from their first two games, their fate is out of their hands.
9.55pmThat's all we've got time for this evening. Please stay with us at Sports Mole as we bring you all of the action from today's final World Cup match, as Japan take on Greece. Until next time, goodbye.