The 32-year-old missed the first two games of the tournament after returning a positive coronavirus test, but he has since participated in full training with the squad and ought to be in contention to feature here.
It would not be a surprise to see Busquets displace Rodri in the engine room after the Manchester City star picked up a booking in the recent draw with Poland, while Koke and teenage starlet Pedri should keep their places in the middle.
Aymeric Laporte was out-muscled and out-jumped by Robert Lewandowski for Poland's equaliser against La Roja but is expected to continue alongside Pau Torres here, while the versatile Marcos Llorente should continue at right-back.
Dani Olmo did not enjoy the most fruitful of evenings against Poland but will hope to keep his place on the left over Mikel Oyarzabal, while Luis Enrique has a decision to make over Gerard Moreno following his mixed evening on matchday two.
The Villarreal man assisted Alvaro Morata's opener - which was ultimately given following a VAR review - before striking the post with a penalty, but with five goals and four assists for the national team since his debut in October 2019, he will be eyeing a second chance as Ferran Torres warms the bench.
Spain possible starting lineup: Simon; Llorente, P.Torres, Laporte, Alba; Busquets, Koke, Pedri; Moreno, Morata, Olmo