MK Dons have faltered in recent weeks, picking up just one point from their last four league matches, in a run of form that has seen them fall out of the League One playoff picture.
David Flitcroft has worked miracles at Oakwell since he took over as manager last month, leading his side to five victories in their last six matches.
He has also taken the Tykes to the brink of climbing out of the Championship relegation zone and he will be confident of taking his side into the last eight of the FA Cup.
Flitcroft could name the same starting lineup that began last week's 3-2 win over Middlesbrough.
MK Dons: WWLLLD
Possible starting lineups
MK Dons: Martin; Otsemobor, Lewington, MacKenzie, Williams; Potter, Powell, Harley, Chadwick; Bowditch, Baldock
Barnsley: Steele; Golbourne, Foster, Cranie, Kennedy; Delap, O'Brien, Perkins; Dagnall, Harewood, Etuhu
Sports Mole says: 1-3