The Barcelona attacker lasted just 30 minutes as a substitute against Hungary before succumbing to a knee injury, and he is now confirmed to be missing the rest of the tournament.
However, Dembele was never likely to earn a start up top in place of Karim Benzema or Kylian Mbappe, even though both attackers were guilty of wasting some gilt-edged chances in the recent draw with Hungary.
Antoine Griezmann scored Les Bleus' equaliser on the day and can now boast 11 goals at major tournaments for his country, which places him fourth in the list behind Michel Platini, Just Fontaine and Thierry Henry.
Benjamin Pavard has been in the wars at Euro 2020 and was controversially cleared to play at the weekend after his head injury versus Germany, but Jules Kounde could push for a start if the Bayern Munich man is spared.
France possible starting lineup: Lloris; Kounde, Varane, Kimpembe, Hernandez; Kante, Pogba, Tolisso; Griezmann; Mbappe, Benzema