The Spanish champions booked their spot in the round of 16 courtesy of a 2-1 win over Ibiza on January 22.
No team has won the Copa del Rey on more occasions than Barca, although the Catalan giants lost 2-1 to Valencia in last season's final.
Here, Sports Mole rounds up Barca's latest injury and suspension news ahead of the match.
Type of injury: Thigh
Possible return date: February 15 (vs. Getafe)
Ousmane Dembele has been on the sidelines since picking up a thigh problem during the Champions League clash with Borussia Dortmund in November. The 22-year-old, who has struggled with injuries throughout his time at Camp Nou, is making good progress in his recovery, though, and should return next month.
Type of injury: Knee
Possible return date: Unknown
Luis Suarez underwent an operation earlier this month after damaging the external meniscus on his right knee. The Uruguay international is out for the foreseeable future, although it is thought that he will be available for selection before the end of the 2019-20 campaign.
Status: Major doubt
Type of injury: Ankle
Possible return date: January 30 (vs. Leganes)
Neto, who played the full 90 minutes against Ibiza in the last round of the Copa del Rey, was not involved against Valencia on Saturday afternoon due to an ankle sprain. The club's number two goalkeeper has not been officially ruled out of this match, although the Brazilian faces a fight to prove his fitness.
BARCELONA'S SUSPENSION LIST
Barca have no players suspended for the Copa del Rey clash on home soil.