However, the Catalan giants have only won once in their last four games, while also requiring a penalty to see off Real Valladolid at the weekend.
Lionel Messi got on the scoresheet in that game, and the Argentine will want to add to his 30 goals for the season when taking to the pitch against the Ligue 1 side.
Ahead of this week's meeting, Sports Mole assesses how Messi has fared in his previous outings against Lyon.
Messi has previously faced Lyon on four occasions, but it has been 10 years since the attacker went up against the French side.
The teams have not locked horns since the last-16 stage in 2009, and Messi contributed just one goal as Barca emerged victorious by a 6-2 scoreline on aggregate.
There were also two encounters during the previous campaign, with Messi netting once in each of the group-stages games during the 2007-08 season.
That means that Messi has a total of three strikes from 357 minutes of football against Lyon, with Barcelona registering two wins and two draws from those meetings.