Anderlecht secured a comfortable 2-0 win against 10-man Galatasaray thanks to a brace from defender Chancel Mbemba Mangulu but both sides have been eliminated after Arsenal beat Borussia Dortmund in the other Group D fixture.
Anthony Vanden Borre had his appeals for a penalty dismissed by Croatian referee Ivan Bebek, before Steven Defour saw his mishit volley strike both posts after rolling agonisingly across the goal line.
Anderlecht took the lead just before half time when defender Mbemba headed home from six yards out after Mitrovic's smart flick-on.
However, their hopes of finding a leveller were extinguished on 83 minutes when Selcuk Inan was dismissed for a second bookable offence.
With a man advantage, Anderlecht wrapped up the win when Mbemba reacted quickest to fire home the lose ball after Fernando Muslera had spilled a shot.
The victory means that the Belgian side will drop into the Europa League as Galatasaray finish bottom of Group D.