The 28-year-old has been drawn against world number six Angelique Kerber in the opening round.
Despite facing a tough opponent, Keothavong has insisted that she is "ready" to perform on Court 17.
"Been chilling out this afternoon in NY," she wrote on Twitter. "Ready to play and have some fun tomorrow @usopen. 2nd match on Court 17."
Keothavong has never gone beyond round three at Flushing Meadows.