Result: Serena Williams stunned at French Open

Serena Williams in action against Jelena Jankovic during their WTA Championships match on October 26, 2013
© Getty Images
Defending champion Serena Williams is beaten in the second round of the French Open by world number 35 Garbine Muguruza.

Defending champion Serena Williams has crashed out in the second round of the French Open against world number 35 Garbine Muguruza.

The defeat marks the second time in three years that the world number one has failed to get past the second round at Roland Garros.

The American, who was seeded first, struggled to find her rhythm during the opening set as the Spaniard broke twice on her way to closing it out 6-2.

Williams, usually so assured in her serving, was broken a further three times by Muguruza in the second before the 20-year-old sealed the surprise win 6-2 6-2.

The loss means that it is the first time in women's Grand Slam singles history that the top two seeds have not reached the second round following Li Na's defeat to Kristina Mladenovic on Tuesday.

Serena Williams in action against Sloane Stephens during their US Open third round match on September 1, 2013
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2Spain Rafael Nadal7,555
3Switzerland Roger Federer7,145
4Switzerland Stanislas Wawrinka5,780
5Serbia Novak Djokovic5,325
6Croatia Marin Cilic5,155
7Alexander Zverev4,470
8Austria Dominic Thiem4,030
9Japan Kei Nishikori3,285
10Canada Milos Raonic3,230
11Bulgaria Grigor Dimitrov3,070
12France Jo-Wilfried Tsonga2,770
13Belgium David Goffin2,560
14Spain Roberto Bautista Agut2,425
15Czech Republic Tomas Berdych2,390
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19United States John Isner2,110
20United States Sam Querrey2,060