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Miley, who claimed gold in Thursday's 400m medley, is in the reckoning to become a double Commonwealth champion after winning her heat this afternoon.
O'Connor, who also breezed through her qualifier, will be looking to go one better after winning no fewer than three silvers at this year's tournament.
The pair face off with Australia's Alicia Coutts, a seven-time champion at the Games.
Tonight's final is scheduled to begin at 8.09pm.