The 24th anniversary of the 1989 tragedy, which claimed the lives of 96 Liverpool fans during a crush at Sheffield Wednesday's home stadium, falls two days after the match at the Madejski stadium.
A statement on the club's official website said: "Supporters of Reading Football Club and Liverpool Football Club will hold a minute's silence before Saturday's fixture at Madejski Stadium as a mark of respect for the anniversary of the Hillsborough disaster.
"With the game falling two days before the 24th anniversary of the tragedy, plans began last week for a fitting tribute to the 96 supporters who lost their lives. The Royals contacted Liverpool FC; spoke to the Premier League earlier today and they of course agreed it was absolutely correct to pay respect on such an occasion."
Meanwhile, Reading chairman John Madejski has revealed that he would personally opt for a minute's silence to pay respect to Baroness Thatcher, who passed away on Monday.