The Spanish legend, who died in May last year, was renowned for the blue and white attire that became synonymous with his five Major victories.
Captain Jose Maria Olazabal - a great friend of Ballesteros - has had the team's bags emblazoned with Ballesteros's famous 1984 Open Championship-winning pose.
Ian Poulter believes that his memory can inspire Europe to a famous victory.
"We have Seve's blue and white, Seve on the bag and his right-hand man as our captain [can] hopefully see us home," said Ian Poulter.
USA lead 10-6 heading into the singles matches.