The Americans drew first blood in the day's second match, taking the lead on the second hole when Garcia had struck an iron well past the green.
Donald nearly sank a snaking long-range putt at the fifth hole for an eagle, but it circled the perimeter of the hole before spewing out.
Mickelson had the chance to halve the hole with a 15-footer, but sent it wide and Europe were back on level terms regardless.
Donald then sunk a tough putt on the next hole to turn the match on its head.
Garcia found the green on the ninth hole, but had left Donald with a tricky put from around 15 feet. Once again, Donald was denied by the rim of the hole.
Meanwhile, Mickelson had dialled in a superb wedge shot that left Bradley with a five-foot putt to win the hole. He duly sank it and that match is all square.