After a halved hole on the first, Johnson found an accurate 7-iron on the par-three second to set up a birdie that the Europeans couldn't equal.
The third was halved with pars before Colsaerts, the hero of yesterday's fourballs for Europe, missed a short putt at the fourth to hand the home pair a 2-up lead.
Dufner and Johnson couldn't get away, however, making a bogey on six to give the Europeans hope of bringing the match back level.
Seven and eight were halved before Garcia found a tidy approach to the ninth and Colsaerts tickled in the birdie. It wasn't good enough to bring the match back all square though as Dufner's putt just crept in.