Macclesfield produced one of the biggest shocks of recent years by beating Cardiff at Moss Lane in the third round, and Martinez believes that their home advantage could prove to be effective once more.
The Wigan boss pointed to his side's third round clash with Bournemouth - in which they required a replay to progress - as proof that lower league sides raise their games when playing Premier League sides.
"There is going to be a lot of passion in the Moss Rose ground on Saturday and it is going to be a typical FA Cup tie. Divisional status is not an issue in the FA Cup or any cup for that matter and as we've seen against Cardiff City in the last round, Macclesfield use their home ground to their advantage and they were very effective against the leaders of the Championship, which is a phenomenal achievement," said Martinez.
"As we saw ourselves against Bournemouth in the last round, players can raise their game in one-off matches like these and they can hit extraordinary physical levels which bring about an extra difficulty when you are trying to prepare.
"We're going to have over 2,000 of our fans filling a third of the ground which makes it even more significant for us and in many ways it is a blast from the past for some of our supporters that have been following the club since our days in the lower divisions. We are really looking forward to the game and we want to progress to the fifth round."
Wigan are currently 19th in the Premier League table, while Macclesfield are ninth in the Conference.