The 56-year-old has guided the Friulani to Champions League qualifcation in back-to-back seasons following his move from Parma in 2010.
However, Guidolin revealed that his is in need of a break from football in order to concentrate on his health.
"I am very tired and need to rest," Guidolin told Sky Sport Italia. "I think I'm going to need a long rest, as I am under a lot of strain and I don't know if my health can hold up to another season like this with over 50 matches.
"In the next few days I will talk to the directors, because I feel the need to go off grid and stop for a while, so I don't know if I'll be ready for the start of July."
The Italian has hinted that he may stay on in a less stressful role, such as becoming a technical director at the club.
"Could I stay in Udine as technical director working with a young coach?" he added. "I would be ready for that role."
Guidolin has previously taken Vicenza to the Cup Winners' Cup semi-final in 1998, where they lost to Chelsea.