Spurs make the trip to France armed with a 2-1 victory from the opening leg at White Hart Lane last week.
However, Lyon are still considered to have a decent chance of progression because they have an away goal to their name.
As a result, Parker has conceded that it will be a "tough" encounter for his side in Eastern France.
"We know it will be tough. They are well organised with a wealth of Champions League experience and some very good players," Parker told the club's official website.
"We'll need to be at our best to get a result but we've showed our quality away from home this season and we're hoping to put in another positive performance."
The winners of the tie will player either Inter Milan or CFR Cluj in the next round.