Puel has suggested that he will make alterations to the team which beat Everton on Sunday afternoon, but he insists that his players should still be able to put in a high-level performance in the last-eight clash at the Emirates Stadium.
The 55-year-old told reporters: "It's an important game. It's an opportunity to try to win something. All my squad is ambitious.
"The last game against Everton was a fantastic game, with good intensity and a good physical level. Every time we can rotate the team and keep the same philosophy. Arsenal are the same. It will be a good game of football."
Southampton are attempting to reach the semi-finals of the competition for the first time since 1987.