The Italian tactician believes that Destro, who was on Inter's books between 2005 and 2010, will show his class at the San Siro.
"Miralem Pjanic is not well and I don't think he will be part of the squad tomorrow, though we've still got a day to decide," Andreazzoli told Roma Channel on Tuesday.
"[Daniele] De Rossi is coming off seven or eight days in which he has done very little training. The situation is improving and we'd be happy to have him at our disposal. At this stage of the season, I think Inter are not the only side to have injury problems. We have them too, but won't sit here listing them.
"We rested Destro against Torino precisely so we could give him three or four more days to train and prepare. This will be Destro's game for many reasons, including his return from injury, his past with Inter and the importance of the fixture."
Roma won the first leg 2-1 at the Stadio Olimpico in Rome.