The Portuguese will replace Marcelo, who will be out of action for the next two weeks with a hamstring injury.
It had been speculated that Gareth Bale, who started his career as a left-back, could feature in Marcelo's position, but Ancelotti has quelled such claims.
"Coentrao has experience for this game and he has fresh legs to replace Marcelo. We have Nacho, [Raphael] Varane and [Sergio] Ramos to play in the back row. Bale is not an option for this position," Ancelotti told reporters at a press conference.