The two met in rain-soaked Portugal a fortnight ago, with the referee choosing not to abandon the game in which Benfica grabbed a late equaliser to draw 1-1.
This week's meeting at the Karaiskakis Stadium will go a long way to deciding who qualify for the knockout stages, probably as runners-up to Paris Saint-Germain in Group C.
Both teams have four points going into the contest, where Benfica striker Oscar Cardozo will attempt to continue his run of scoring in each of his last four matches.
Olympiacos are in fine form themselves, having gone eight games unbeaten since taking a 4-0 hammering at the hands of PSG in September.
Argentine forward Javier Saviola was on the bench against his former club two weeks ago, but may be given a start up front if the Greek outfit opt to play a more attacking 4-4-2 formation on home soil.
Possible starting lineups
Olympiacos: Roberto; Maniatas, Holebas, Manilas, Leandro; Samaris, Ndinga, Siovas, Weiss; Saviola, Mitroglou
Benfica: Moraes; Luisao, Garay, Siqueira; Gaitan, Almeida, Matic, Cavaleiro, Perez; Cardozo, Lima
Sports Mole says: 1-1