Wigan are currently in the Premier League relegation zone, but they are unbeaten in their last four games, winning three of them.
Kenny Jackett's Championship outfit come into the match having not won in their last three games, but the Lions will be hoping to upset the odds against their top-flight opponents.
Millwall are likely to make changes to the side which lost 2-1 to Sheffield Wednesday in midweek, with Liam Trotter and James Henry in contention to start, while on-loan striker Jermaine Easter is cup-tied.
Possible starting lineups
Millwall: Forde; Dunne, Shittu, St Ledger, Lowry; Beevers, Trotter, Chaplow, Henry; Keogh, Hulse
Wigan: Robles; Alcaraz, Boyce, Scharner, Figueroa; McCarthy, Maloney, Gomez, Beausejour, McManaman; Kone
Sports Mole says: 1-2