The Turkish side overcame Viktoria Plzen in the previous round to advance to this stage of the competition, while Lazio were comfortable winners over Stuttgart.
Fenerbahce will enter the match full of confidence having won four of their last five matches in all competitions, a run of form that has left them in second position in their domestic table, just four points behind leaders Galatasaray.
Lazio, however, have won just two of their last six matches in Serie A, with that disappointing run of form dropping them into fifth position in the table.
The home side could name the same team that overcame Akhisar Bld. at the weekend, with former Liverpool pair Dirk Kuyt and Raul Meireles both starting. Miroslav Stoch is also pushing for inclusion, but is expected to start on the bench.
Possible starting lineups:
Fenerbahce: Demirel; Kormaz, Irtegun, Ziegler; Meireles, Baroni, Topuz, Erkin; Sow, Kuyt, Webo
Lazio: Marchetti; Biava, Rady, Stankevicius, Onazi; Gonzalez, Hernanes, Ledesma, Cana; Saha, Klose
Sports Mole says: 1-1