The Trotters have recovered from a 10-game winless run at the start of the season to climb the table, but Barnsley remain deep in relegation trouble.
They are five points adrift of safety, although they have played a game fewer than all of their rivals, except for Charlton Athletic, ahead of their trip to the Reebok Stadium on Saturday afternoon.
However, having previously had the division's worst away record, the Tykes won at Reading and Yeovil Town in their last two matches on the road and may approach the game with cautious optimism.
Jim O'Brien will not feature again this season, and while there is better news on the fitness of David Fox and 20-year-old forward Marcus Pedersen, neither is expected to be in the squad this weekend.
The hosts are likely to continue with two up front in the form of Joe Mason, who has notched in successive games, and Lukas Jutkiewicz. The 4-4-2 system probably leaves no room for top scorer Andre Moritz.
Possible starting lineups
Bolton: Bogdan; Hutton, Wheater, Knight, Ream; Chung-Yong, Medo, Spearing, Danns; Mason, Jutkiewicz
Barnsley: Steele; Etuhu, Crainie, Nyatanga, Kennedy; Jennings, Cwyka, Lawrence, Dawson; McCourt; O'Grady
Sports Mole says: 2-0