The Irishman shocked many when he abruptly left the Midlands club in August 2010 after four years in charge.
The team that he comes up against on Saturday is in stark contrast to the one he departed two years ago as Villa are hoping to secure their Premier League survival.
With one win in their last six, Alex McLeish's side have been dragged into a relegation battle.
However, three points against the Black Cats would see them all but book their place in the top flight for next season.
Marc Albrighton, Chris Herd, Ciaran Clark and Stephen Warnock are all expected to return for the hosts, while O'Neill has hinted that he may throw some younger players into the team for the remaining few games of the campaign.
Aston Villa: WLLDDL
Aston Villa: Given; Hutton, Collins, Clark, Warnock; Albrighton, Herd, Ireland, Bannan; Agbonlahor, Weimann
Sunderland: Mignolet; Bardsley, Turner, Kilgallon, Richardson; Larsson, Gardner, Vaughan, McClean; Sessegnon, Bendtner
Sports Mole says: 0-1