The forward picked up an ankle problem during the Villans' 2-0 defeat at home to Everton last weekend.
Agbonlahor will now be assessed by the club's medical staff before the team makes the trip to Upton Park.
Lambert told Sky Sports News: "He's got a knock on that ankle that was giving him a bit of a problem before so we have to wait and see. Everybody else seems not too bad."
Villa are currently in 13th place in the Premier League table with 10 points from their opening nine matches.