The Blues meet their London rivals on Saturday just days before their Champions League semi-final second leg against Barcelona at the Nou Camp.
"We will have to assess the players but there is a good chance some fresh ones will come in," said Di Matteo.
"Also, they deserve to be in the team as well. I left a few players out against Barcelona because I have to make decisions.
"We will see about Saturday but I have it in mind to give one or two a start."