The Seagulls earned a 2-0 victory over Newcastle United in the third round and a home tie with Arsene Wenger's team represents an opportunity for them to test themselves against another of the Premier League's top sides.
Manager Gus Poyet wasn't satisfied with his team's display in their 1-1 draw with Blackburn Rovers in midweek but he may hand the players who started that match a chance to redeem themselves against the Gunners.
Wenger is expected to make several changes to the side that defeated West Ham United 5-1 on Wednesday night.
Brighton & Hove Albion: LWWWDD
Possible starting lineups:
Brighton & Hove Albion: Kuszczak; Greer, El-Abd, Calderon, Bridge; Orlandi, Vicente, Dicker, David Lopez, Bridcutt; Barnes
Arsenal: Szczesny; Jenkinson, Mertesacker, Koscielny, Santos; Rosicky, Ramsey, Cazorla; Oxlade-Chamberlain, Arshavin, Giroud
Sports Mole says: 1-3